Isolation, Identification and application of oil degrading bacteria for bio remediation of oil pollution

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Anirban Hembram

Abstract

Bio remediation is the use of biological sources, especially bacteria and fungi for the clean up of the environment and prevention of pollution. Selection of oil degrading bacteria in the current investigatory research was solely for the purpose of oil spill and oil contamination cleaning from the environment which may include water bodies, land forms or any other natural habitat. These habitats especially the water bodies if untreated may cause death of many aquatic and terrestrial life's disturbing the ecological balance of nature. In the current work it was aimed to isolate and identify the oil consuming bacteria which can potentially convert the oil polymers into simple monomers for their metabolism and prevent the accumulation of these oils in the natural habitats. The source selected for the insolation was based on the highest chances of obtaining positive cells. Thus the study included the use of soil sample from an oil refinery near the laboratory. Bacteria were isolated from the source sample and screened for the presence of positive cultures capable of oil degradation using minimal salt agar media. Positive cultures were used for test oil degradation and assay. The bacteria showing positive for hydrocarbon degradation were identified using basic biochemical tests followed by sequencing of the 16s ribosomal RNA. Positive cultures thus obtained can be efficiently used in the bio-remediation of oil contamination in water bodies and other environment where they act as pollutants. The final confirmation of oil degradation was performed by inoculating the bacterial cells into BMH media supplemented with oil and estimating the reduction in oil content after 2 weeks volumetric ally. This can serve as a qualitative assay for the conformation of oil degrading cultures. 

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How to Cite
Hembram, A. . (2022). Isolation, Identification and application of oil degrading bacteria for bio remediation of oil pollution. Helix - The Scientific Explorer | Peer Reviewed Bimonthly International Journal, 12(2), 1-7. Retrieved from https://helixscientific.pub/index.php/home/article/view/394
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