Extraction, Purification and Testing of Anti Diabetic Activity of Secondary Metabolites from Calotropis

Abstract

Diabetes being a life threatening disease of the 19^th and 20^th century  affecting 2 out of every 5 individuals needs an attention for the development of promising treatment protocols. Being a hereditary disease makes the count far more than the genetic and external factors for the disease.  The worst part of diabetes is the lack of cure and the fact that it can only be managed by life style and diet changes. Thus adapting a healthy lifestyle and use of anti diabetic food and herbs improves the condition of the victim. The current work involves the study of anti diabeticactivity of Calotropis leaf and flower extracts. Solvents like ethanol, water and chloroform were used to prepare the  extracts from plant based samples. These extracts were tested for their efficacy against blood glucose by measuring their inhibition against the vital enzymes in glucose absorption alpha amylase and glucosidase.  The parameter used to check the anti diabetic activity was inhibition of alpha amylase and alpha glucosidase by the extracts. They act as references for the anti diabetic activity of the samples.