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Use of microbes as bioreactors for the industrial production of several metabolites and products of commercial importance have been one of the prominent applications in science and technology. Especially industrial scale production of alcohol using Yeast Saccharomyces cerevicie has been the practice since long and yet most demanded. In view of the commercial importance of alcohol production by yeast, the current work aimed to analyze several inoculation and incubation conditions like, temperature, pH, Chemicals etc on the growth and production of alcohol by baker’s yeast. The yeast required is obtained from a grocery store which acts as a seed culture for the production of mother plate followed by its use in the inoculation of alcohol production media. The effect of various growth conditions on the growth and alcohol production were studied using spectrophotometry. Based on the statistics the optimal conditions for high growth and good enzyme production were calibrated.
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